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Casalini
Stefano


Contact Data

Phone: + 34 935 801 853
Ext: 346
Fax: + 34 935 805 729
E-mail: [javascript protected email address]
Dep: Molecular Nanoscience and Organic Materials
ResearcherID

Bio

By checking my curriculum, one can get the following chronological overview of my career:

1)      01/09/1998 - 18/07/2003             Graduation in chemistry

2)      01/02/2004 - 01/01/2005             Pre-doctoral grant funded by Vinicola San Nazario s.r.l.

3)      01/01/2005 - 19/02/2008             PhD in chemistry

4)      15/06/2008 - 14/01/2009             Post-doctoral grant funded by Tetrapak s.p.a.

5)      15/04/2009 - 14/04/2012             Post-doctoral research grant funded by ONE-P European project

6)      15/04/2012 - 15/04/2015             Post-doctoral research grant funded by I-ONE European project

7)      01/06/2015 - 15/12/2015             Visiting researcher at ICMAB-CSIC

8)      16/12/2015 - 15/03/2016            Post-doctoral research grant funded by e-GAMES ERC grant

9)      16/03/2016 - 15/03/2018            TECNIOSpring2015 fellow cofounded by Marie Curie Programme.

 

As schematically listed, my career has not experienced any remarkable break, even though I changed different working places such as University of Modena and Reggio Emilia, two companies (viz. Vinicola San Nazaro and Tetrapak), Institute for Nanostructured Materials (ISMN-CNR) and Institut de Ciència de Materials de Barcelona (ICMAB-CSIC). Along the scientific education of a student, the degree graduation and the PhD defense are two natural breaks in his career. In my case, they coincided to two collaborative experiences with two industries (Vinicola San Nazaro s.r.l. and Tetrapak s.p.a.). These two contracts allowed me to make use of my scientific skills in a non-academic environment giving me the opportunity to work in the field of applied science together with their R&D teams. My first Post-Doc grant was funded by the European project “Organic Nanomaterials for Electronics and Photonics (ONE-P)”. My second Post-Doc grant was funded by the European project “Implantable Organic NanoElectronics (I-ONE)”. The former was mainly focused on Molecular Electronics, whereas the latter one on Bio-Electronics. Recently, I got the TECNIOSpring2015 fellowship with total budget equal to 133020 €. Finally, I could state that my career is well balanced by looking carefully at each skill that an independent researcher must need.

i)                     I showed a good flexibility by moving my research topic three times.

ii)                   I worked in two large European projects, in which I was responsible of different activities (e.g. I was responsible of several deliverables and milestones). This allowed me to work for more than 6 years at the top level in Europe.

iii)                  I published 25 peer-reviewed articles and one book chapter. I was 1st author in 14 of them, once as last author and 11 times as corresponding author. I had more than 55 co-authors. This clearly shows my attitude to manage several national and international collaborations, and it also hints to my degree of independence on developing idea together with their scientific dissemination through talks and/or peer-reviewed articles.

iv)                 I was part of the organizing committee of three different International Conferences and I co-supervised several students both under-graduate and PhD.

The synergy between my expertise and the scientific background of Dr. Marta Mas-Torrent has yielded to the fabrication of a robust platform well-suited for bio-applications. This allowed me to publish 2 peer-reviewed papers in a very short time as well as to get funding for that (i.e. TECNIOSpring2015 fellowship, total budget 133020 €).

 

Doctorate, University and Year:

I got the PhD at the University of Modena and Reggio Emilia under the supervision of Prof. M. Sola.

Title: “Engineering cytochrome c for electrochemical biosensing applications

The work basically consists in the change of the wild-type cytochrome c to a peroxidase-like protein by means of genetic engineering. Two type of mutants have been produced: M80A/C102T and M80A7N62C/C102T, which are lacking of the sixth metal ligand (viz. methionine 80). The production of these two new proteins is based on protein engineering, cell expression along with production and protein purification. The adsorption of these proteins onto Au electrode is the central core of the amperometric biosensors. In particular it has been proved the electrocatalytic reduction of molecular oxygen mediated by M80A/C102T and M80A7N62C/C102T mutants. In the last part of the PhD, other two new proteins have been produced, such as M80A/Y67H/C102T and M80A/Y67A/C102T, whose electrical properties aim to a wider range of analyte of interest, as nitrite ion and hydrogen peroxide. 

Keywords

electrochemistry, organic electronics, surface engineering

Indicators

Number Articles: 27
Number Citations: 321
Hfactor: 11

Scientific Highlights

As clearly shown in my curriculum, I worked in the following three research areas: i) bio-electrochemistry, ii) molecular electronics and iii) bio-electronics. Although these three topics forced me to reset three times my research activity, my career has been always focused on (bio-)organic materials deployed to electrical tests. Regarding to the first area (namely my PhD thesis), I genetically modified a metallo-protein, Cytochrome c, in order to get a bio-catalytic core well-suited for amperometric sensors. As a result, I succeeded to electro-catalyze important targets such as oxygen, hydrogen peroxide and nitrite ions. Within this context, I published 4 papers: i) Journal of American Chemical Society (as 1st author); ii) 2 Journal of Physical Chemistry B (as 1st author in both) and iii) Journal of Electroanalytical Chemistry (as 4th author). Concerning my second scientific area, my 1st Post-Doc grant was funded by the European project “Organic Nanomaterials for Electronics and Photonics (ONE-P)”. This was one of the largest project funded by the European commission (total budget >27M€) bringing together 28 partners spread all over Europe. The research activity was mainly focused on the study of the charge-injection across self-assembled monolayers once implemented in the organic field-effect transistors. I ended up with 10 peer-reviewed papers (like Chemical Society Reviews, Chemical Communications, Applied Physics Letters, Journal of Materials Chemistry, Langmuir etc.) and one book chapter (editor Wiley). Regarding to the third scientific area, my second Post-Doc grant was funded by the European project “Implantable Organic NanoElectronics (I-ONE)”. My research activity dealt with two parallel activities: i) the development of a biosensor capable to detect low levels of cytokines and ii) testing materials compatible for implantation. From these activities, I published 9 peer-reviewed papers such as ACS Applied Materials and Interfaces, ACS Nano, Analytical Chemistry etc.

Recently, I got the TECNIOSpring2015 grant (total budget: 133020€) from the Government of Catalunya and co-funded by the Marie-Curie programme. The project title is "HI-TECH platform for label-free biosensors". The project deals with the development of a potentiometric sensor capable to detect specific biomarkers related to neurological diseases such as Alzheimer. Two peer-reviewed papers have been already published in Advanced Materials and Scientific Reports.

To resume my research line, I had a strong expertize on two types of electrical tests: i) electrochemistry and ii) organic transistors. I exploited these two strategies to a wide range of (bio-)organic compounds. I demonstrated how self-assembling molecules can be exploited to tune the charge-injection in organic transistors, or to be implemented in a planar array of electrodes for water actuation. Furthermore, a potentiometric sensor was developed to detect dopamine. This particular work has been recently awarded from the editorial board of Organic Electronics, as one of the manuscript most cited since its publication up to June 2016.  Regarding to engineered  proteins, amperometric sensors were demonstrated as well as immuno-sensor to detect O2/H2O2/NO2 and cytokines respectively.

 
 
 
 

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